Carbol Fuchsin

Carbol Fuchsin

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Carbol Fuchsin

Carbol Fuchsin is a red dye widely used in microbiological staining, particularly in the Ziehl-Neelsen method for acid-fast bacteria.

Carbol Fuchsin is a deep red solution used extensively in microbiology for staining acid-fast bacteria, such as Mycobacterium species. It is a crucial component of the Ziehl-Neelsen stain and the Kinyoun stain, helping to differentiate bacteria based on the lipid content of their cell walls.
  • Chemical Composition: Mixture of Basic Fuchsin and phenol
  • Appearance: Dark red solution
  • Solubility: Soluble in water and alcohol
  • Boiling Point: Approx. 100�C (212�F)
  • pH: Typically around 7.0 for staining solutions
  • Storage: Store in a tightly closed container in a cool, dry place, away from light
    • Applications

    Microbiology: Essential in the Ziehl-Neelsen and Kinyoun staining techniques for identifying acid-fast bacteria, such as Mycobacterium tuberculosis.

    Histology: Occasionally used for staining certain tissue components to enhance contrast.

    Usage Instructions

    Preparation: Carbol Fuchsin is typically provided as a ready-to-use solution. Ensure it is properly mixed before use.

    Staining Protocol

    Ziehl-Neelsen Method: Heat fix the bacterial smear, apply Carbol Fuchsin, heat gently, and then rinse.

    Kinyoun Method: Apply Carbol Fuchsin to the fixed smear without heating, let it stand for the specified time, and then rinse.

    Decolorization and Counterstaining: Follow with acid-alcohol decolorization and counterstain with methylene blue or brilliant green to differentiate acid-fast bacteria from non-acid-fast bacteria.

    Safety Information

    Carbol Fuchsin contains phenol, which is toxic and can cause burns. Handle with care, avoiding inhalation, ingestion, and contact with skin and eyes. Use appropriate personal protective equipment (PPE), such as gloves, lab coat, and safety goggles. Work in a well-ventilated area or under a fume hood.

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